| Congresso Brasileiro de Microbiologia 2023 | Resumo: 874-2 | ||||
Resumo:Klebsiella pneumoniae and Pseudomonas aeruginosa are Gram-negative bacilli (GNB) known by their high incidence in healthcare-associated infections. Carbapenems are the main therapeutic choices in the treatment of infections caused by multidrug-resistant GNB. The spread of carbapenemase-producing GNB on hospital settings is a major health concern, as the World Health Organization points out that K. pneumoniae and P. aeruginosa resistant to carbapenems are pathogens of critical priority. The aim of this study was to evaluate the production of carbapenemases in clinical isolates of K. pneumoniae and P. aeruginosa obtained from patients at two hospitals in the state of Paraíba, Northeastern Brazil, between January to October 2022. Identification at specie-level was performed by MALDI-TOF and antibiotic susceptibility profile were carried out through the automated system VITEK 2®, following the protocols of the Brazilian Committee on Antimicrobial Susceptibility Testing (BrCAST). The detection of blaKPC, blaNDM, blaGIM, blaVIM, blaSPM and blaIMP genes were carried out by PCR. A total of 37 carbapenem-resistant isolates were obtained. From 24 K. pneumoniae, 4.2% (1/24) were Multidrug-Resistant (MDR), 50% (12/24) Extensively Drug-Resistant (XDR) and 45.8% (11/24) Pandrug-Resistant. Resistance to meropenem and imipenem ranged from 91.7% (22/24) to 95.8% (23/34), respectively. Amikacin had the lowest incidence of resistance, 45.8% (11/24). Regarding the susceptibility results of P. aeruginosa isolates, 15.4% (2/13) were classified as MDR, 30.8% (4/13) XDR and 53.8% (7/13) PDR. All P. aeruginosa isolates were resistant to imipenem and 84.6% (11/13) to meropenem. The blaKPC gene was identified in 40.5% (15/37) of the isolates, blaNDM gene in 32.4% (12/37) and blaVIM gene in 10.8% (4/37). Co-occurrence of blaKPC and blaNDM genes was observed in 8.3% (2/24) of K. pneumoniae isolates and co-occurrence of blaKPC and blaVIM genes in 30.8% (4/13) of P. aeruginosa. All VIM-producing isolates were detected in P. aeruginosa harboring blaKPC gene. Isolates carrying blaGIM, blaSPM and blaIMP genes were not detected. Our results highlight that continuous surveillance is essential to monitor the spread of clinically important GNB co-producing carbapenemase in the Northeast region of Brazil. The dissemination of this isolates could lead to a worrisome scenario. Palavras-chave: Gram-negative bacilli, Pandrug-Resistant, Epidemiological analyzes Agência de fomento:Instituto Nacional de Pesquisa em Resistência Antimicrobiana - INPRA (INCT – MCTI/CNPq/CAPES/FAPs nº 16/2014) | |||||